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Article Details

  • Article Code : FIRAT-AKADEMI-9250-5551
  • Article Type : Araştırma Makalesi
  • Publication Number : 5A0186
  • Page Number : 257-267
  • Doi : 10.12739/NWSA.2022.17.4.5A0186
  • Abstract Reading : 950
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Issue Details

  • Year : 2022
  • Volume : 17
  • Issue : 4
  • Number of Articles Published : 15
  • Published Date : 1.10.2022

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Dergi Kapağı
Ecological Life Sciences

Serial Number : 5A
ISSN No. : 1308-7258
Release Interval (in a Year) : 4 Issues

DİKENLİ TATLISU YILAN BALIĞI (Mastacembalus mastacembalus, Bank ve Solander, 1794)’IN SOLUNGAÇLARININ HİSTOLOJİK YAPISI VE BAZI HİSTOKİMYASAL ÖZELLİKLERİ

Hatice Eda DABAK1 , SİBEL KÖPRÜCÜ2

Çalışmada; Elazığ Keban Baraj Gölü’nden temin edilen 5 adet Dikenli Tatlısu Yılan Balığı (Mastacembalus mastacembalus, Bank ve Solander, 1794)’nın solungaçlarının histolojik ve histokimyasal özellikleri ışık mikroskobunda incelendi. Mastacembalus mastacembalus’un solungaçları primer filament ve sekonder lamelları olan her biri iki hemibranştan oluşan dört holobranştan şekillenmiştir. Solungaç filamentleri afferent ve efferent arterlerle vaskularizedir. Filament ve lamellada, plaster, klorid ve mukus hücreleri bulunmaktadır. Klorid hücreleri az sayıda olup, primer filamentte yer almaktadır. Mukus hücreleri, primer filamentte ve sekonder lamellanın tabanında bol miktarda görülmektedir. Solungaçlarda yapılan histokimyasal boyamalar sonucunda mukus hücrelerindeki glikokonjugatlar; güçlü sülfatlı (AB pH 0.4) negatif, O-esterli (AB pH 1.0) pozitif, asidik (AB pH 2.5) kuvvetli pozitif, nötral (PAS) pozitif, nötral ve asidik (PAS/AB pH 2.5) asidik baskın, sülfatlı (AF) negatif, sülfatlı asidik (AF/AB pH 2.5) asidik baskın, siyalik asit rezidülü (saponifikasyon) (KOH/PAS) negatif reaksiyon gösterdi.

Keywords
Mastacembalus mastacembalus, Solungaç, Histoloji, Yapı, Histokimya,

INVESTIGATION OF HISTOLOGY STRUCTURE AND SOME HISTOCHEMICAL CHARACTERISTICS OF GILLS IN THE SPINY EEL, (Mastacembalus mastacembalus, Bank and Bolander, 1794)

Hatice Eda DABAK1 , SİBEL KÖPRÜCÜ2

In this study, the histological structure and histochemical characteristics of gills of five spiny eel, (Mastacembalus mastacembalus, Bank and Solander, 1794)’s gills obtained in Keban Dam lake were examined in the light microscope. The gills of Mastacembalus mastacembalus are formed by four holobranchs, each consisting of two hemibranchs with primary filaments and secondary lamellae. The gill filaments are vascularized by afferent and efferent arteries. The filament and lamellae contain plaster, chloride and mucous cells. Chlorid cells are scarce in the primary filaments. Mucous cells are observed abundant on the primary filament and secondary lamellae. As a result of histochemically staining of the gills of Mastacembalus mastacembalus were determined that mucous cells included strong sulphate (AB pH 0.4), O-sulphate esters (AB pH 1.0), acidic (AB pH 2.5), neutral (PAS), neutral and acid rich (PAS/AB pH 2.5). Mucous cells containing sulphate and acidic (AF/AB pH 2.5) showed dominant acid reaction. Mucous cells including sulphate (AF) and sialic acid recidues (KOH/PAS) were seen negative reaction.

Keywords
Mastacembalus mastacembalus, Gill, Histology, Structure, Histochemistry,

Details
   

Authors

Hatice Eda DABAK (1)

edadabak@firat.edu.tr | 0000-0002-4142-2221

SİBEL KÖPRÜCÜ (2) (Corresponding Author)
FIRAT ÜNIVERSITESI
skoprucu@firat.edu.tr | 0000-0002-6565-3550

Supporting Institution

 : Araştırma Fırat Üniversitesi Bilimsel Araştırma Projeleri Birimine [INJ]SÜF.15.01 nolu proje[INJ]tarafından desteklenmiştir.

Project Number

 : SÜF.15.01 nolu proje

Thanks

 : Yazarlar araştırmayı “SÜF.15.01 nolu proje” ile destekleyen “Fırat Üniversitesi Bilimsel Araştırma Projeleri Birimine” teşekkür eder.
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